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Relationship Between Polymorphisms of Interleukin-1 Receptor Antagonist (IL-1Ra) Genes and Susceptibility to Systemic Lupus Erythematosus in Iranian Population

[ Vol. 16 , Issue. 2 ]

Author(s):

Rashin Ganjali, Jalil T. Afshari, Zahra Rezaieyazdi*, Mandana Khodashahi, Azam Brook, Maryam Mazhani, Mohamad R. Hatef and Mahnaz Abbasi   Pages 105 - 109 ( 5 )

Abstract:


Objectives: Interleukin (IL)-1 has a major role in cell destruction and inflammation. IL-1 receptor antagonist (IL-1RN or IL-1Ra) is a natural anti-inflammatory molecule that blocks IL-1. We intended to determine whether IL-1RN or IL-1Ra variable number tandem repeat (VNTR) polymorphism is associated with susceptibility to systemic lupus erythematosus (SLE) in a series of patients in the Northeastern part of Iran.

Methods: Genomic DNA was extracted from the whole blood of 104 SLE patients and 209 subjects without SLE as a control group. The control group was matched for age and gender with SLE patients. Then, genomic DNA was genotyped by polymerase chain reaction (PCR) method for a length polymorphism in intron 2 of the IL-1RN gene.

Results: Of five alleles, only allele 4 of IL-1RN had a higher frequency in healthy subjects (2.4%) compared to SLE patients (0), with a statistically significant difference (P= 0.03). Eleven kinds of polymorphisms of IL-1RN were found including 1/1, 1/2, 2/2, 3/3, 1/3, 3/5, 2/3, 2/5, 1/5, 4/4 and 1/4 in both groups. In genotype frequency, there was no statistically significant difference regarding gene polymorphism kinds between the two groups (P= 0.29).

Conclusion: Alleles 4 of IL-1RN may have a protective role against SLE susceptibility. However, SLE was not associated with any of the 11 kinds of genotype IL1-RN gene polymorphisms studied here.

Keywords:

Systemic lupus erythematosus, interleukin-1, receptor, antagonist, genes, polymorphism, gene polymorphism.

Affiliation:

Immunogenetic and Cell Culture Lab, Immunology Center, Bu-Ali Institute, Mashhad University of Medical Sciences, Mashhad, Immunogenetic and Cell Culture Lab, Immunology Center, Bu-Ali Institute, Mashhad University of Medical Sciences, Mashhad, Rheumatic Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Rheumatic Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Immunogenetic and Cell Culture Lab, Immunology Center, Bu-Ali Institute, Mashhad University of Medical Sciences, Mashhad, Immunogenetic and Cell Culture Lab, Immunology Center, Bu-Ali Institute, Mashhad University of Medical Sciences, Mashhad, Rheumatic Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Metabolic Diseases Research Center, Qazvin University of Medical Sciences, Qazvin

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